Characterization of plasmid pAW63, a second self-transmissible plasmid in Bacillus thuringiensis subsp. kurstaki HD73
نویسندگان
چکیده
منابع مشابه
Complete Genome Sequence of Bacillus thuringiensis subsp. kurstaki Strain HD73
Bacillus thuringiensis is a Gram-positive bacterium that produces intracellular protein crystals toxic to a wide variety of insect larvae. We report the complete genome sequence of Bacillus thuringiensis subsp. kurstaki strain HD73 from the Centre OILB (Institut Pasteur, France), which belongs to serotype 3ab and is toxic to lepidopteran larvae.
متن کاملReplication mechanism and sequence analysis of the replicon of pAW63, a conjugative plasmid from Bacillus thuringiensis.
A 5.8-kb fragment of the large conjugative plasmid pAW63 from Bacillus thuringiensis subsp. kurstaki HD73 containing all the information for autonomous replication was cloned and sequenced. By deletion analysis, the pAW63 replicon was reduced to a 4.1-kb fragment harboring four open reading frames (ORFs). Rep63A (513 amino acids [aa]), encoded by the largest ORF, displayed strong similarity (40...
متن کاملChanges in Physiological Properties as a Criterium for Detection of Plasmid Loss in Bacillus thuringiensis
Recent technological improvements have extended the application range of dielectric permittivity biomass measurements to on-line monitoring of physiological changes during bacterial fermentations. In an industrial fermentation of Bacillus thuringiensis, it is important to verify the intactness of bacterial plasmid content in all steps of culture. Changes in certain plasmid resident properties o...
متن کاملFacile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki.
We describe a method for maximizing the rate of conversion of Bacillus thuringiensis subsp. kurstaki vegetative cells to osmotically fragile forms in the absence of exogenously added enzymes. Optimal generation of autoplasts occurred in 50 mM sodium acetate buffer (pH 7.0) at 37 degrees C with 10% (wt/vol) polyethylene glycol as an osmotic stabilizer. The maximum autolytic rate resulted in a co...
متن کاملGenome Sequence of Bacillus thuringiensis subsp. kurstaki Strain HD-1
We report here the complete genome sequence of Bacillus thuringiensis subsp. kurstaki strain HD-1, which serves as the primary U.S. reference standard for all commercial insecticidal formulations of B. thuringiensis manufactured around the world.
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ژورنال
عنوان ژورنال: Microbiology
سال: 1998
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-144-5-1263